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Because HDAC1 was overexpressed in resistant H1975, PC9/IR, and HCC827/IR cells and its knockdown could sensitize resistant cells to gefitinib, the effect of WJ-26210-2 combined with gefitinib on these resistant cells was examined. Lncap-derived and PC3-derived cell lines were maintained in RPMI-1640 (Invitrogen) supplemented with 10% FBS and 1% penicillin-streptomycin in a 5% CO2 cell culture incubator.